ABSTRACT
In this work, we used dual-polarization interferometry to explore the binding events between adenosine triphosphate (ATP) and ATP-binding aptamer (ABA) at solid-liquid interface.The single-stranded ABA was immobilized onto the chip surface.After the addition of ATP, the real-time and label-free technique for detailed investigation of their interactions were reflected on the changes of the mass, thickness, and density through DPI.By analysis of the binding curves from changes in mass, the association rate constant (ka, 4.66×10.3 L/(mol·s), dissociation rate constant (kd, 1.70 × 10.-2 s.-1), dissociation constant (KA, 2.7 × 10.5 L/mol), and association constant (KD, 3.7 × 10.-6 mol/L) were precisely determined.Moreover, good linear correlations between ATP concentrations and three parameters (mass, thickness, density) resolved by the response to ATP binding were obtained.The detection limits (LOD, 3σ) were 0.22 μmol/L for mass calibration, 0.14 μmol/L for thickness calibration, and 0.32 μmol/L for density calibration.We expect that this DPI-based aptasensor can be utilized to study the interactions of functional ABA with ATP, and also can be used for the detection of ATP with high sensitivity.
ABSTRACT
The 13 nm gold nanoparticles ( AuNPs ) were synthesized through the reduction of HAuCl4 by sodium citrate and the glutathione was assembled on the AuNPs. Under the experiment conditions, glutathione-modified AuNPs ( GSH-AuNPs ) with negative charge presented a wine red color owing to the electrostatic repulsion between nanoparticals. Upon the addition of neurogenin 3 ( ngn3 ) peptide, the aggregation of GSH-AuNPs was induced by ngn3 peptide under a certain concentration of salt. The color of AuNPs solution changed from red to blue as a function of the ngn3 peptide concentration. Thus, a rapid detection method for ngn3 peptide using GSH-AuNPs as colorimetric probe was established. The optimal experiment conditions were equilibrium time=10 min, pH=6. 0, CNaCl=100 mmol/L. Under the optimum conditions, the assay showed a linear response range of 20-300 μg/L for the peptide with a detection limit being 8 μg/L and exhibited excellent selectivity for ngn3 peptide.
ABSTRACT
Objective To scrutinize the CT features of pulmonary actinomycosis and analysize the causes of misdiagnosis.Methods CT images of eleven patients with pulmonary actinomycosis proved by pathology were retrospectively reviewed.Among them,6 were scanned with additional enhanced CT scan.Results Among the 11 patients,the lesions located in the inferior lobe in 7 patients,and in the superior lobe in 4 patients.The lesions affected two or more pulmonary segments in 9 patients,6 of them involving two lobes.Irregular mass or nodules with necrotic low-attenuation areas were the main signs in all patients,and segmental consolidations were found in 6 patients.Peripherally progressive enhancement was found in all patients with contrast enhanced CT scan.Vicinity pleural thickening was found in 11 patients,while extrapleural fat deposition was found in 6 patients.One case showed erosion of the chest wall and rib.Conclusion Pulmonary actinomycosis is a chronic suppurative granulomatous inflammation,which is often misdiagnosed with neoplasm or other lung disease because of its mass-like performance.The peripherally progressive enhancement on CT has a certain diagnostic value for P\pulmonary actinomycosis.
ABSTRACT
Objective:To investigate the clinical effects of deanxit in the treatment of post-stroke depression and anxiety.Method:65 stroke patients with depressive and anxious disorder were randomly divided into two groups which were treated with deanxit(as deanxit group) and without deanxit(as controlled group) respectively.SDS and SAS scales were used to evaluate the changes of clinical symptoms.Result:The scores of SDS and SAS scales in the deanxit group after 2 weeks'treatment were 36.37?7.07 and 32.91?5.28,which were significantly lower than those of the controlled group (P